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KMID : 0384119910110030567
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Korean Journal of Clinical Pathology
1991 Volume.11 No. 3 p.567 ~ p.574
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Characterization of Acute Leukemia Through Automatic Assessment of Peripjeral, Blood Cells by USE of Technicon H-1
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Y. S. Y ang
H. J. Kang/W. Hsong/H. I. Cho/S. I. Kim
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Abstract
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The Technicon H-1 is a hematology autoanalyzer that performs complete blood cell count and differential count of white blood cells. This analyzer permits assessment of the patient`s clinical status through a combination of numeric results, morphology flags, cytograms, and histograms. We evaluate the usefulness and unique capabilities of this instrument in the characterization of acute leukemias. Forty one cases of acute leukemia were studied. The sensitivity of this system for blast flag was 93%. There was no correlation between the large unstained cell % of this system and blast % in manual differential count. The 17 cases of acute lymphoblastic leukemia(ALL) had significant differences in percentage of lymphocyte, monocyte, neutrophil, large unstaind cell( LUC), and basophil, mean peroxidase index(MPXI), and percentage of postitive flag for left shift, compared with 24 cases of acute myelocytic leukemia( AML). In all of ALL cases, LUC % was greater than 4%, neutrophil % was lower than 40%, and monocyte % was lower than 3.4%. Therefore, all cases presented H(high) flags for LUC %, L(low) flags for monocyte and neutrophil %. The H flags for lymphocyte % were positive in 15 cases of ALL. In AML cases, H flags for LUC % were presented only in 50%, but positive flags for left shift and abnormal MPXI flags were presented in 54.2 % and 70.8%, which were not appeared in ALL cases at all. The L flags for monocyte that is presented in 100% of ALL were positive only in 4 cases of AML. In stead, the H flags for monocyte were positive in 15 cases of AML. This combination of high or low signals in differential count, morphology flags, and MPXI permited the differential diagnosls of ALL and AML. Subtypes of AML could not be absolutely distinguished, but promyelocytic leukemta(M3) could be presumptively dragnosed with characteristic cytogram and high MPXI score. Mean monocyte % of M4 and M5 cases was higher than that of Ml and M2, but was not appropriate as diagnostic criteria. Conclusively, since Information is readily avatlable by using this automatic assessment, we suggest that interpretation of reports by H-l 1s useful m the rapid diagnosis and classification of acute leukemias.
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